Conference on methods and applications in the frontier between MX and CryoEM

19-21 September 2017 | Barcelona, Spain

About the conference

Structural biology lends eyes to the life sciences. Currently, it is undergoing a revolution with the extension of single particle cryo electron microscopy (CryoEM) to atomic resolution, previously the realm of macromolecular crystallography (MX). Both CryoEM and MX methods are rapidly evolving, blending to rise to the new challenges.
This conference will be timely, joining developments in the frontier of MX and CryoEM, with applications in Biomedicine.

REGISTRATION DEADLINE

25 May 2017

SELECTED PARTICIPANTS WILL BE NOTIFIED NO LATER THAN

30 May 2017

PAYMENT DEADLINE

30 June 2017

Organizing Committee

Isabel Usón

Structural Biology Unit, Barcelona

ICREA Research Professor

Garib Murshudov

Laboratory of Molecular Biology, Cambridge

Programme

19 September

8:30      Participants registration

9:00      Welcome by Isabel Usón

9:10      Introduction by Garib Murshudov

Session I – Overview and principles

9:15      Richard Henderson (LMB-MRC, Cambridge, UK)

“An overview of cryoEM”

10:00    Sjors Scheres (LMB-MRC, Cambridge, UK)

“A Bayesian view on cryo-EM structure determination”

10:45    Coffee and poster presentation

11:15    José María Valpuesta (CNB-CSIC, Madrid)

“Combining biochemical and biophysical techniques and low-resolution EM maps for the generation of quasi-atomic models: the case of molecular chaperone complexes”

12:00    Holger Stark (MPIBPC, Göttingen, Germany)

“How to overcome biochemical limitations in sample preparation for high-resolution structure determination of macromolecular complexes by cryo-EM and X-ray crystallography”

12:45    Lunch

Session II – Solution and reconstruction

15:00    Airlie McCoy (CIMR, University of Cambridge, UK)

“Solving X-ray crystal structures with cryo-EM reconstructions”

15:45    Randy Read (CIMR, University of Cambridge, UK)

“Repurposing molecular replacement algorithms for docking models in Cryo-EM reconstructions”

16:30    Coffee and poster presentation

17:15    Paul Emsley (LMB-MRC, Cambridge, UK)

“Model-building in Low Resolution and Cryo-EM maps using Coot”

18:00    Tristan Croll (CIMR, University of Cambridge, UK)

“ISOLDE: interactive model building in a physically realistic environment”

18:45    End of the day

 

20 September

Session III – Model Building to automation

9:15      Jon Agirre (YSBL, University of York, UK)

“Automated model building in cryoEM maps”

10:00    José María Carazo (CNB-CSIC, Madrid)

“New approaches to the analysis of cryoEM maps quality”

10:45    Coffee and poster presentation

11:15    Elena Orlova (University of London, UK)

“Structural studies of heterogeneous assemblies”

12:00    Tom Terwilliger (Los Alamos National Laboratory, USA)

“Model-building using cryo-EM and crystallographic maps “

12:45    Lunch

Session IV – Refinement and Global Initiatives

15:00    Garib Murshudov (LMC-MRC, Cambridge, UK)

“Refinement of atomic models into cryo-EM maps using REFMAC5”

15:45    Pavel Afonine (Lawrence Berkeley National Laboratory, Berkeley CA, USA)

“New real space refinement methods for cryo-EM”

16:30    Coffee and poster presentation

17:15    Tom Burnley (CCPEM, UK)

“Introducing the Collaborative Computational Project for Electron cryo-Microscopy: CCP-EM”

18:00    Paula da Fonseca (LMB-MRC, UK)

“The emerging role of CryoEM in drug discovery and development”

18:45    End of the day

 

21 September

Session V – Application and Future prospects 

9:15      Werner Kühlbrandt (Max-Plank Institut für Biophysik)

“Investigating membrane protein structure and function by cryo-EM”

10:00    José Castón (CNB-CSIC, Madrid)

“Cryo-EM at near-atomic resolution of icosahedral viruses”

10:30    Coffee and poster presentation

11:15    Abhay Kotecha (Division of Structural biology, University of Oxford, UK)

“CryoEM illuminates the world of the Picornaviruses”

11:45    Rafael Fernandez Leiro (CNIO, Madrid)

“Mechanistic insights on DNA replication and repair by cryo-EM”

12:15    Paola Fucini (CIC-Biogune, Bilbao)

“Structural studies of functional ribosome complexes”

13:00    Concluding remarks

13:15    End

Registration

REGISTRATION DEADLINE

25 May 2017

SELECTED PARTICIPANTS WILL BE NOTIFIED NO LATER THAN

30 May 2017

PAYMENT DEADLINE

30 June 2017

REGISTRATION FEE

Academic: 150€

Non academic: 300€

Registration includes:

Participation

Morning coffee break, lunch, afternoon coffee break

Venue

Contact

email: mx-cryoem-bcn@ibmb.csic.es

Organizers

Organization Collaborator

Sponsors

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Generalitat

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